Composite

Part:BBa_K1084502:Design

Designed by: Shinya Kawata   Group: iGEM13_HokkaidoU_Japan   (2013-09-26)


Promoter Selector-aeBlue


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 213
    Illegal SpeI site found at 455
    Illegal SpeI site found at 673
    Illegal PstI site found at 687
    Illegal PstI site found at 1746
    Illegal PstI site found at 1880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 455
    Illegal SpeI site found at 673
    Illegal PstI site found at 228
    Illegal PstI site found at 687
    Illegal PstI site found at 1746
    Illegal PstI site found at 1880
    Illegal NotI site found at 221
    Illegal NotI site found at 680
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 214
    Illegal SpeI site found at 455
    Illegal SpeI site found at 673
    Illegal PstI site found at 687
    Illegal PstI site found at 1746
    Illegal PstI site found at 1880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 455
    Illegal SpeI site found at 673
    Illegal PstI site found at 228
    Illegal PstI site found at 687
    Illegal PstI site found at 1746
    Illegal PstI site found at 1880
    Illegal AgeI site found at 70
    Illegal AgeI site found at 529
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 73
    Illegal BsaI site found at 532
    Illegal BsaI.rc site found at 67
    Illegal BsaI.rc site found at 526


Design Notes

coming soon


Source

coming soon

References